QUANTITATIVE ASPECTS OF THE TRICARBOXYLIC ACID CYCLE IN BAKER'S YEAST

Quantitative aspects of the tricarboxylic acid cycle in baker's yeast.

The quantitative significance of the tricarboxylic acid cycle in the oxidation of acetate by yeast has remained in doubt despite many investigations concerning this topic. All of the enzymes of the tricarboxylic acid cycle have been demonstrated in either commercial baker's yeast or in pure cultures of Saccharomyces cerevisiae. (Weiland and Sonderhoff, 1932; Lynen and Neciullah, 1939; Novelli a...

Activities of tricarboxylic acid cycle enzymes, glyoxylate cycle enzymes, and fructose diphosphatase in bakers' yeast during adaptation to acetate oxidation.

Bakers' yeast oxidizes acetate at a high rate only after an adaptation period during which the capacity of the glyoxylate cycle is found to increase. There was apparently no necessity for the activity of acetyl-coenzyme A synthetase, the capacity of the tricarboxylic acid cycle, or the concentrations of the cytochromes to increase for this adaptation to occur. Elevation of fructose 1,6 diphosph...

Tricarboxylic Acid Cycle in

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...

The Tricarboxylic Acid Cycle in Dictyostelium discoideum

Experimental studies of enzyme kinetics in vitro and metabolic fluxes in vivo have been used by Wright and her colleagues to develop a detailed kinetic model of the tricarboxylic acid cycle in Dictyostelium discoideum. This model has recently been been analyzed by two different methods (Albe, K. R., and Wright, B. E. (1992) J. Biol. Chem. 267, 3106-3114; Shiraishi, F., and Savageau, M. A. (199...